The efficacy of hyaluronidase diluted with anesthetic lidocaine in the degradation of hyaluronic acid

Vol. 5 – Number 18 – 2024 Original article Page 48-58 The efficacy of hyaluronidase diluted with anesthetic lidocaine in the degradation of hyaluronic acid Julliana Cunha de Almeida¹ ABSTRACT Objective: The study aims to analyze whether products applied to Biogelis Volume hyaluronic acid cause its degradation, as well as to investigate whether 1 minute is sufficient for the gel to transition to the liquid state. Secondary objectives include identifying methods of diluting hyaluronidase, evaluating the role, advantages, and disadvantages of enzyme use, and contributing to scientific knowledge about the enzymatic degradation of hyaluronic acid (AH) at different dilutions. Methods: A rheological study of Biogel Volume AH exposed to dilutions of hyaluronidase was conducted, using lidocaine without vasoconstrictor or sterile saline solution. Samples were analyzed in triplicate using a rotational rheometer to determine the storage modulus (G’). Results: All diluents affected the degradation of hyaluronic acid within 1 minute, with significant variation only in the elastic modulus (G’) among the diluents. Conclusions: The choice of diluent can selectively impact the elastic modulus of the gel, while other properties may not be affected. Dilution with lidocaine combined with saline showed greater significance compared to dilution with saline alone or with the anesthetic alone. Descriptors: Hyaluronic acid, hyaluronidase, viscoelasticity, degradation. ¹ Bucomaxilofacial; Esp., Me. e Doutoranda em Harmonização Orofacial. DOI: 10.24077/2024;5183207787815